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Merck
모든 사진(1)

주요 문서

11922416001

Roche

Anti-His6

from mouse IgG1

동의어(들):

metal chelate affinity chromatography

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모든 사진(1)

About This Item

UNSPSC 코드:
12352203

생물학적 소스

mouse

Quality Level

100

결합

unconjugated

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

monoclonal

양식

lyophilized

포장

pkg of 100 μg

제조업체/상표

Roche

동형

IgG1

저장 온도

2-8°C

일반 설명

Anti-His6 is a monoclonal antibody to His6-tagged proteins. Anti-His6 specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the location of the histidine-tag in the protein sequence.

특이성

Anti-His6 specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the histidine-tag in the protein sequence. In addition, Anti-His6 preferentially recognizes the C-terminal His6-epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.

애플리케이션

Anti-His6 is used for the detection of His6-tagged proteins in:
  • Immunoblotting (e.g., dot blots and western blots)
  • Immunoprecipitation
  • Immunoassays (ELISA)
  • Immunocytochemistry
  • Immunofluorescence

제조 메모

Stabilizers: BSA
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • ELISA: for detection 0.1 μg/ml; for coating 1 to 5 μg/ml
  • Immunoprecipitation: 0.5 to 5 μg/ml
  • Western blot: 0.2 to 0.5 μg/ml

재구성

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.

기타 정보

For life science research only. Not for use in diagnostic procedures.

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픽토그램

Exclamation mark
GHS07

신호어

Warning

유해 및 위험 성명서

H317,H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
70058800
54579800
47120300
44850200
40670900

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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