추천 제품
생물학적 소스
mouse
결합
peroxidase conjugate
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
1A4, monoclonal
양식
lyophilized powder
분자량
antigen ~42 kDa
종 반응성
frog, mouse, hamster, chicken, viper, bovine, crayfish, goat, rat, cat, sheep, guinea pig, lizard, human, rabbit, dog, snail
기술
direct ELISA: suitable
immunoblotting: 2-4 μg/mL using extracts of mouse heart tissues
immunohistochemistry: 5-10 μg/mL using human appendix or tonsil tissue sections
동형
IgG2a
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... ACTA2(59)
일반 설명
Monoclonal Anti-Actin, α-Smooth Muscle-HRP (mouse IgG2a isotype) is derived from the hybridoma 1A4 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with a synthetic peptide corresponding to a sequence of α-smooth muscle actin, conjugated to KLH. Actin is a highly conserved protein, expressed as six isoforms and represents differentiation markers of muscle tissues.
면역원
a synthetic peptide corresponding to a sequence at the NH2 terminal synthetic decapeptide of α-smooth muscle actin, conjugated to KLH.
애플리케이션
Monoclonal Anti-Actin, α-Smooth Muscle - Peroxidase antibody has been used in:
- direct-immunoblotting
- direct-immunohistochemistry
- direct- enzyme linked immunosorbent assay (ELISA)
- direct staining of tissues and cells
- characterization of stromal cell heterogeneity in various organs and distinguish smooth muscle cells from fibroblasts in mixed cultures.
생화학적/생리학적 작용
Actin and Myosin are two major cytoskeletal proteins implicated in cell motility, both constitutively expressed in many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. It has been shown that relative proportions of actin isoforms are diverse in smooth muscles of different organs and change within the same population of smooth muscle cells during development, pathological situations and different culture conditions.
물리적 형태
Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline
제조 메모
Reconstitute in 0.1mL of distilled water.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
이미 열람한 고객
E M Abd-el-Basset et al.
Neuroscience letters, 125(2), 117-120 (1991-04-29)
In mouse astroglia colony cultures, cells display dramatic changes in their 'social behavior' during differentiation. The non-motile glioblasts progress through highly motile pro-astroblast and astroblast stages before becoming non-motile fibrous astrocytes. These changes in the behavior of astroglia are paralleled
Niels van Royen et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 16(3), 432-434 (2002-02-01)
Increased expression of transforming growth factor beta1 (TGF-beta1) during collateral artery growth, as well as its numerous effects on monocytes/macrophages and the smooth muscle cell cycle and differentiation, suggest a modulating role for this growth factor during arteriogenesis. We studied
A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
Skalli O, et al.
The Journal of Cell Biology, 103(6), 2787-2796 (1986)
Stephanie Kern et al.
FEBS open bio, 4, 11-17 (2013-12-10)
We previously reported that a restrictive N-terminal truncation of cardiac troponin I (cTnI-ND) is up-regulated in the heart in adaptation to hemodynamic stresses. Over-expression of cTnI-ND in the hearts of transgenic mice revealed functional benefits such as increased relaxation and
Ryuji Morizane et al.
Scientific reports, 4, 4578-4578 (2014-04-04)
micro RNAs (miRNAs) are small non-coding RNAs that act as posttranscriptional repressors by binding to the 3'-UTR of target mRNAs. On the other hand, mesenchymal-epithelial transition (EMT) and kidney fibrosis is a pathological process of chronic kidney disease (CKD), and
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.