추천 제품
생물학적 소스
mouse
Quality Level
결합
CY3 conjugate
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
1A4, monoclonal
형태
buffered aqueous solution
분자량
antigen ~42 kDa
종 반응성
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
저장 조건
protect from light
기술
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix
동형
IgG2a
UniProt 수납 번호
응용 분야
research pathology
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Monoclonal Anti-Actin, α-Smooth Muscle (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice.
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.
면역원
N-terminal synthetic decapeptide of α-smooth muscle actin.
애플리케이션
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (2 papers)
Immunocytochemistry (3 papers)
Immunofluorescence (13 papers)
Immunohistochemistry (27 papers)
Western Blotting (2 papers)
Flow cytometry/Cell sorting (2 papers)
Immunocytochemistry (3 papers)
Immunofluorescence (13 papers)
Immunohistochemistry (27 papers)
Western Blotting (2 papers)
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
생화학적/생리학적 작용
Smooth muscle actin is transiently coexpressed with sarcomeric α-actin during myogenesis in chicken and rat embryos. It has been found in the ventricular conducting tract of adult mammalian heart. It is expressed in leiomyomas, leiomyosarcomas and leiomyoblastomas, as well as in a proportion of rhabdomyosarcomas.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.
기타 정보
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
법적 정보
Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Coexpression of alpha-sarcomeric actin, alpha-smooth muscle actin and desmin during myogenesis in rat and mouse embryos I. Skeletal muscle
Babai F, et al.
Differentiation, 44(2), 132-142 (1990)
Jinhua Li et al.
The American journal of pathology, 175(4), 1380-1388 (2009-09-05)
Diabetic nephropathy is the leading cause of chronic renal failure. Myofibroblasts play a major role in the synthesis and secretion of extracellular matrix in diabetic renal fibrosis. Increasing evidence suggests that endothelial cells may undergo endothelial-myofibroblast transition under physiological and
C Konoeda et al.
Transplantation proceedings, 45(5), 1797-1801 (2013-06-19)
Aberrant epithelial repair is a crucial event in the airway remodeling that characterizes obliterative bronchiolitis (OB) in transplanted lungs. Recent data from experiments using epithelial cell lines and human airway tissues from lung transplant recipients suggest that epithelial to mesenchymal
Debby Gawlitta et al.
Tissue engineering. Part A, 18(1-2), 208-218 (2011-08-24)
To ensure the survival of engineered bone after implantation, we combined human endothelial colony forming cells (ECFCs) and multipotent stromal cells (MSCs) as a proof of concept in a co-culture model to create in vitro prevascularized bone constructs. We hypothesized
Patricia Parsons-Wingerter et al.
The American journal of pathology, 167(1), 193-211 (2005-06-24)
Integrin alpha5beta1 is among the proteins overexpressed on tumor vessels and is a potential target for diagnostics and therapeutics. Here, we mapped the distribution of alpha5beta1 integrin in three murine tumor models and identified sites of expression that are rapidly
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