추천 제품
형태
lyophilized powder
Quality Level
특이 활성도
≥1,000 units/mg protein
분자량
39.5 kDa
구성
Protein, ≥70% biuret
저장 온도
−20°C
유전자 정보
Escherichia coli CFT073 ... sodA(1040208) , sodB(1036179) , sodC(1036143)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide. The direct electron transfer of superoxide dismutases can be efficiently promoted by a self-assembled monolayer of 3-mercaptopropionic acid confined on a gold electrode.
애플리케이션
Superoxide dismutase from Escherichia coli has been used in a study to determine that anion binding properties of reduced and oxidized iron-containing superoxide dismutase reveal no requirement for tyrosine 34. Superoxide dismutase from Escherichia coli has also been used in a study to demonstrate that molecular dynamics simulation and limited proteolysis are complementary and specific tools to identify flexible sites in proteins.
생화학적/생리학적 작용
Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.
기타 정보
Manganese-containing enzyme
단위 정의
One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
물리적 형태
Contains Tris buffer salts
분석 메모
For assay method, see McCord, J.M. and Fridovich, I., J. Biol. Chem., 244, 6049 (1969).
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Yang Tian et al.
Analytical chemistry, 76(14), 4162-4168 (2004-07-16)
In this article, the electrochemical properties and electrocatalytic activity of three kinds of superoxide dismutases (SODs), that is, bovine erythrocyte copper-zinc superoxide dismutase (Cu/Zn-SOD), iron superoxide dismutase from Escherichia coli (Fe-SOD), and manganese superoxide dismutase from E. coli (Mn-SOD), in
M Falconi et al.
Proteins, 47(4), 513-520 (2002-05-10)
Limited proteolysis by trypsin of monomeric Cu,Zn superoxide dismutase from Escherichia coli induces a specific cleavage of the polypeptide chain at the level of Lys60 located in the S-S subloop of loop 6,5 where, when compared to the eukaryotic enzyme
Anne-Frances Miller et al.
Biochemistry, 44(16), 5969-5981 (2005-04-20)
We report the first spectroscopic observation of substrate analogue binding to the reduced state of iron superoxide dismutase from Escherichia coli (Fe(2+)SOD) and demonstrate that the pH dependence reflects inhibition of anion binding by ionized Tyr34, not loss of a
David G Westbrook et al.
Cardiovascular toxicology, 10(3), 216-226 (2010-07-30)
Epidemiological studies suggest that events occurring during fetal and early childhood development influence disease susceptibility. Similarly, molecular studies in mice have shown that in utero exposure to cardiovascular disease (CVD) risk factors such as environmental tobacco smoke (ETS) increased adult
Vera G Grivennikova et al.
Redox biology, 17, 192-199 (2018-04-28)
The initial rates of superoxide plus hydrogen peroxide (ROS) generation by intact or permeabilized rat heart mitochondria and coupled inside-out bovine heart submitochondrial particles (SMP) oxidizing NAD-dependent substrates, NADH, and succinate were measured by detecting resorufin formation in the Amplex
프로토콜
Enzymatic Assay of Superoxide Dismutase
Separation of Superoxide dismutase
Chromatograms
application for HPLC자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.