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Merck
모든 사진(1)

주요 문서

R7397

Sigma-Aldrich

ProtectRNA RNase Inhibitor 500× Concentrate

RNase inhibitor for in situ hybridization assays

동의어(들):

Ribonuclease inhibitor ProtectRNA

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About This Item

UNSPSC 코드:
41106300
NACRES:
NA.52

Quality Level

양식

liquid

저장 온도

2-8°C

일반 설명

Not recommended in systems where other enzymatic activity is required.

ProtectRNA ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.

애플리케이션

A potent inhibitor of most nucleic acid binding enzymes, and thus useful as an RNase inhibitor. Especially useful when performing in situ hybridization. If it is added to all aqueous solutions used, it eliminates the need for special glassware washing and after-wash treatments. The 500x concentrate is economical; 2 ml treats 1,000 ml of solution. Not recommended in systems where other enzymatic activity is required.
ProtectRNA RNase Inhibitor 500× Concentrate has been used in:
  • RNA extraction
  • tissue sectioning and staining for laser capture microscopy
  • the isolation of blood vessels from hippocampi
ProtectRNA RNase Inhibitor 500× Concentrate is an RNase inhibitor.

특징 및 장점

  • The 500× concentrate is economical
  • Eliminates the need for special dishwashing and“after washing” cleaning and treatments.

수량

2mL treats 1,000ml of solution.

기타 정보

This product is for R&D use only, not for drug, household, or other uses.

법적 정보

ProtectRNA is a trademark of Sigma-Aldrich Co. LLC

픽토그램

FlameCorrosion

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Flam. Liq. 3 - Met. Corr. 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point (°F)

140.0 °F - closed cup

Flash Point (°C)

60 °C - closed cup


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