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Merck
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Key Documents

M1028

Sigma-Aldrich

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

동의어(들):

MgCl2, dichloromangesium, magnesium chloride

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About This Item

Linear Formula:
MgCl2
CAS Number:
Molecular Weight:
95.21
MDL number:
UNSPSC 코드:
12352302
PubChem Substance ID:
NACRES:
NA.52

Grade

for molecular biology

Quality Level

무균

non-sterile; 0.2 μm filtered

형태

liquid

농도

1.00 M±0.01 M

기술

DNA sequencing: suitable

불순물

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

solubility

water: soluble

density

1.080 g/cm3

양이온 미량물

heavy metals (as Pb): ≤5 ppm

적합성

suitable for electrophoresis
suitable for molecular biology

응용 분야

cell analysis

외래 활성

DNase, RNase, NICKase and protease, none detected

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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관련 카테고리

일반 설명

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

애플리케이션

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -
  • to prepare buffer to lyse cells for immunoprecipitation
  • as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.
  • in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.
  • It has been used to protect the liver from oxidative damage.
  • to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

특징 및 장점

  • Available as a Ready-to-use solution which reduces the need for preparation time.
  • Heavy metals (as Pb): ≤5 ppm
  • Free from DNase, RNase, NICKase and protease

성분

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves


시험 성적서(COA)

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문서

Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.

프로토콜

This procedure may be used for Mutanolysin products.

Enzymatic Assay of Fructose-6-Phosphate Kinase Pyrophosphate Dependent

This procedure may be used for all Phosphoglucomutase products except for Phosphoglucomutase, Catalog Number P4109.

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

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