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Merck
모든 사진(1)

주요 문서

M3769

Sigma-Aldrich

Medium 199

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

동의어(들):

M199 Medium, TCM 199

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71

Quality Level

양식

powder

기술

cell culture | mammalian: suitable

성분

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

애플리케이션

Medium 199 has been used:
  • to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
  • to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
  • to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)

수량

Formulated to contain 9.4 grams of powder per liter of medium.

재구성

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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