QPBCA

QuantiPro^™ BCA Assay Kit

for 0.5-30 μg/ml protein

• 동의어(들): BCA Assay, Protein determination by BCA, bicinchoninic acid reagent
• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• Quality Level: 200
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

애플리케이션

In addition to protein determination and quantification in solution, the QuantiPro^™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.

The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

특징 및 장점

• Accurate across a broad range of protein concentrations
• High sensitivity; linear response form 0.5 to 30 μg/ml of protein
• Stable color complex
• Reduced susceptibility to detergents

원리

Based on the alkaline reduction of Cu²⁺ to Cu¹⁺ by proteins, and the formation of a bicinchoninic acid:Cu¹⁺ complex having an absorbance maximum at 562 nm.

저장 및 안정성

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

법적 정보

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

키트 구성품 전용

• Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

키트 구성품 역시 별도로 이용 가능함

• P0914 Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1

안전성 정보

• 픽토그램: GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H290,H315,H318,H410
• 예방조치 성명서: P234 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2
• Storage Class Code: 8A - Combustible corrosive hazardous materials