PP0500

Protease Profiler^™ Kit

Proteases for Mass Spectrometry and Proteomics

• UNSPSC 코드: 12352200
• NACRES: NA.56

속성

• 품질: Proteases for Mass Spectrometry and Proteomics
• 저장 온도: −20°C

설명

일반 설명

For detailed characterization of proteins of interest, increase your protease selection with the Protease Profiler. The Protease Profiler provides four proven alternative proteases in addition to Proteomics Grade Trypsin, an enzyme solublization reagent, and an enzyme reaction buffer. Each component has been stringently purified and qualified for MALDI-MS to ensure optimal performance. Characterize with greater flexibility, perform double enzymatic digestions, and explore alternative cleavage sites with the Protease Profiler.

• Double Enzymatic Digestion - An initial digestion may result in peptide fragments greater than 5 kDa, often above the optimum detection range for most MS systems/instruments. A second digestion using a protease with different specificity results in cleavage of the large peptides, generating smaller peptides suitable for MS.
• Explore Alternative Proteases - Trypsin may be incompatible with some protein samples. Find the protease best suited to your protein of interest and improve the quality of your data.
• Perform Either Solution or Gel-based Digests - Optimized protocols and reagents for both digest conditions are provided, enabling flexibility in your analysis.

애플리케이션

The Protease Profiler^™ Kit can be used for different enzymatic digestions on the proteins of interest.

저장 및 안정성

The entire Protease Profiler^™ Kit retains activity for at least 1 year when stored at –20 °C. Alternatively, some of the components (Trypsin, Lys-C, Asp-N, Trypsin Solubilization Reagent, and Enzyme Reaction Buffer) may be stored at 2–8 °C. The storage temperature indicated on the labels of these products is 2–8 °C. Storage at –20 °C will not compromise the performance of these products.

법적 정보

Protease Profiler is a trademark of Sigma-Aldrich Co. LLC

키트 구성품 전용

• Enzyme Solublization Reagent 1 mL
• Enzyme Reaction Buffer 25 mL

키트 구성품 역시 별도로 이용 가능함

• T6567 Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg
• P3303 Endoproteinase Asp-N from Pseudomonas fragi mutant strain, suitable for protein sequencing, lyophilized powder 2 μg
• P6181 Endoproteinase Glu-C from Staphylococcus aureus V8, suitable for protein sequencing, lyophilized powder 25 μg
• P6056 Endoproteinase Arg-C from mouse submaxillary gland, suitable for protein sequencing, lyophilized powder 5 μg

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H317,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable