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Key Documents

P5381

Sigma-Aldrich

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)

Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)

동의어(들):

D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

bakers yeast

Quality Level

유형

Type III

형태

ammonium sulfate suspension

특이 활성도

≥400 units/mg protein (biuret)

분자량

tetramer 119,500 Da±600

농도

≥3 mg/mL protein Biuret

기술

activity assay: suitable

색상

white

단백질 ID 수납 번호

UniProt 수납 번호

저장 온도

2-8°C

유전자 정보

yeast ... PGI1(852495)

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일반 설명

Research area: Cell Signaling

Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.

애플리케이션

Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:
  • to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.
  • as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
  • to convert fructose to glucose while evaluating fructose content in kudzu roots.

생화학적/생리학적 작용

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

단위 정의

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.

물리적 형태

Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves


시험 성적서(COA)

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문서 라이브러리 방문

S L Lowe et al.
The Journal of biological chemistry, 250(1), 94-99 (1975-01-10)
Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding
Kudzu [Pueraria montana (Lour.) Merr. Variety lobata: A new source of carbohydrate for bioethanol production
Sage RF, et al.
Biomass and Bioenergy, 33, 57-61 (2009)
J Pozueta-Romero et al.
FEBS letters, 291(2), 233-237 (1991-10-21)
The standardized enzyme coupling method for assaying sucrose synthase activities in the direction of sucrose cleavage was reexamined using enzyme preparations from cultured cells of sycamore (Acer pseudoplatanus L.) and spinach leaves (Spinacea oleracea). Both ATP and Tris, commonly utilized
B K Campbell et al.
Reproduction (Cambridge, England), 140(5), 721-732 (2010-08-19)
Glucose is a critical metabolic fuel in most mammals although many foodstuffs also contain high levels of the monosaccharides, galactose and fructose. The aims of this work were to determine the insulin response to challenges of these sugars (experiment 1)
Johannes Yayo et al.
Applied and environmental microbiology, 87(9) (2021-02-21)
The native ability of Clostridium thermocellum to efficiently solubilize cellulose makes it an interesting platform for sustainable biofuel production through consolidated bioprocessing. Together with other improvements, industrial implementation of C. thermocellum, as well as fundamental studies into its metabolism, would

문서

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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