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P1261

Sigma-Aldrich

Phosphorylase a from rabbit muscle

lyophilized powder, 20-30 units/mg protein

동의어(들):

1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase

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About This Item

CAS Number:
9032-10-4
효소 위원회 번호:
2.4.1.1 (BRENDA, IUBMB)
EC Number:
232-878-8
MDL number:
MFCD00167428
UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

rabbit muscle

형태

lyophilized powder

특이 활성도

20-30 units/mg protein

정제법

2× crystallization

구성

Protein, 30-60% E1%/280

외래 활성

AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%
phosphoglucomutase ≤0.25%
phosphorylase b ≤30%

저장 온도

−20°C

일반 설명

Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P).

애플리케이션

Phosphorylase a from rabbit muscle has been used to mix with glycogen for the preparation of limit dextrin. It has also been used to probe the array in oligosaccharide microarray analysis of phosphorylase activity.
Phosphorylase from rabbit muscle has been used in a study to assess the molecular mechanisms of oleanolic acid. It has also been used in a study to describe the conversion of phosphorylase B to A, through a conversion enzyme in the presence of 32P-ATP.

생화학적/생리학적 작용

Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.
Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls.
Phosphorylase participates in glycogenolysis. Deficiency of phosphorylase leads to several glycogen storage diseases with hepatomegaly.

단위 정의

One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30 °C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)

물리적 형태

Lyophilized powder containing β-glycerophosphate and EDTA

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Hypoglycemia in the toddler and child
Pediatric Endocrinology, Diabetes, and Metabolism, 920-955 (2014)
T C Chang et al.
Plant physiology, 80(2), 534-538 (1986-02-01)
A protein, starch phosphorylase inhibitor, was purified from the root of sweet potato (Ipomoea batatas [L.] Lam. cv Tainon 65). It had a molecular weight of 250,000 and could be composed of five identical subunits. The isoelectric point of the
Versatile high-resolution oligosaccharide microarrays for plant glycobiology and cell wall research
Pedersen H L, et al.
The Journal of biological chemistry, M112-M112 (2012)
Maria Kylä-Puhju et al.
Meat science, 69(1), 143-149 (2005-01-01)
The activity of glycogen debranching enzyme (GDE) was studied in relation to pH value and temperature in porcine masseter and longissimus dorsi muscles. A glycogen limit dextrin was used as the substrate for GDE, and the enzyme was derived from
The phosphorylase b to a converting enzyme of rabbit skeletal muscle.
E G KREBS et al.
Biochimica et biophysica acta, 20(1), 150-157 (1956-04-01)
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