P3397

Phosphoglucomutase from rabbit muscle

ammonium sulfate suspension, ≥100 units/mg protein

• 동의어(들): PGM, α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase
• CAS Number: 9001-81-4
• 효소 위원회 번호: 5.4.2.2 (BRENDA, IUBMB)
• MDL number: MFCD00131886
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: rabbit muscle
• Quality Level: 300
• 설명: ammonium sulfate suspension, >=100 units/mg protein
• 양식: ammonium sulfate suspension
• 특이 활성도: ≥100 units/mg protein
• 저장 조건: (Tightly closed)
• 기술: activity assay: suitable
• 색상: white to light yellow
• 외래 활성: lactic dehydrogenase ≤0.5%; phosphoglucose isomerase ≤0.01%; pyruvate kinase ≤0.05%
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Research area: Cell Signaling

Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues.

애플리케이션

Phosphoglycomutase has been used:

• to study glycogenesis and phosphoglycomutase deficiency in humans
• in phosphoglucomutase assays to study metabolic regulation
• for assay A to assay enzymes closely linked to glycogen metabolism in transformed BL21(DE3)C43 cells
• in sucrose synthase (SuSy) andADPglucose pyrophosphorylase (AGPase) assays(3)
• in enzyme activity assay for coupling the reduction of nicotinamide adenine dinucleotide phosphate (NADP⁺)to determine glucose-1-phosphate from sucrose and inorganic phosphate (Pi)

생화학적/생리학적 작용

Phosphoglucomutase(PGM) enzyme plays a vital role in glycolysis and gluconeogenesis. It is involved in glycogen and trehalose metabolism in insects. PGM participates in the development of plants and some microorganisms. It is essential for proteins, lipids, and nucleic acid metabolism and is crucial for the development of plants because glucose-6-phosphate is a crucial central metabolite. PGM catalyzes the interconversion of glucose-6-phosphate (G-6-P)and glucose-1-phosphate (G-1-P).

단위 정의

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

물리적 형태

Crystalline suspension in 3.2 M (NH₄)₂SO₄, pH 6.0 containing 0.01% EDTA

분석 메모

Protein determined by biuret.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)