L4919

Lysozyme from chicken egg white

BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein

• 동의어(들): Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS Number: 12650-88-3
• 효소 위원회 번호: 3.2.1.17 (BRENDA, IUBMB)
• EC Number: 235-747-3
• MDL number: MFCD00131557
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: chicken egg white
• Quality Level: 200
• Grade: for molecular biology
• 제품 라인: BioUltra
• 분석: ≥98% (SDS-PAGE)
• 형태: lyophilized powder
• 특이 활성도: ≥40,000 units/mg protein
• 분자량: single-chain 14.3 kDa
• 구성: Protein, ≥90%
• 기술: cell based assay: suitable
• 적합성: suitable for cell lysis
• 응용 분야: cell analysis
• 저장 온도: −20°C

설명

일반 설명

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

애플리케이션

Lysozyme from chicken egg white has been used:

• in dielectric spectroscopy studies of dynamics of protein
• as a control to measure the human lysozyme activity
• as a supplement in soaking solution to treat lenses

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

생화학적/생리학적 작용

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Lysozymes participate in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

특징 및 장점

• Highly purified by repeated crystallization and dialysis
• Each lot is use-tested for isolation of plasmid DNA from E. coli

단위 정의

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

제조 메모

3× crystallized

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)