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Merck
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L8402

Sigma-Aldrich

Lysozyme from human neutrophils

≥95% (SDS-PAGE), lyophilized powder, ≥100,000 units/mg protein (E1%/280)

동의어(들):

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

CAS Number:
9001-63-2
효소 위원회 번호:
3.2.1.17 (BRENDA, IUBMB)
MDL number:
MFCD00131557
UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

human neutrophils

Quality Level

200

재조합

expressed in E. coli

분석

≥95% (SDS-PAGE)

형태

lyophilized powder

특이 활성도

≥100,000 units/mg protein (E1%/280)

기술

cell based assay: suitable

적합성

suitable for cell lysis

UniProt 수납 번호

P61626

응용 분야

cell analysis

저장 온도

−20°C

InChI

1S/C99H159N37O23/c1-11-50(8)77(132-72(139)46-120-81(145)63(32-33-70(101)137)124-88(152)69-31-21-39-136(69)93(157)68(43-73(140)141)131-84(148)62(29-19-37-116-98(109)110)125-90(154)75(48(4)5)135-91(155)76(49(6)7)133-80(144)57(100)24-16-34-113-95(103)104)92(156)126-60(27-17-35-114-96(105)106)82(146)121-51(9)78(142)129-66(41-54-45-119-59-26-15-13-23-56(54)59)87(151)134-74(47(2)3)89(153)122-52(10)79(143)128-65(40-53-44-118-58-25-14-12-22-55(53)58)85(149)123-61(28-18-36-115-97(107)108)83(147)130-67(42-71(102)138)86(150)127-64(94(158)159)30-20-38-117-99(111)112/h12-15,22-23,25-26,44-45,47-52,57,60-69,74-77,118-119H,11,16-21,24,27-43,46,100H2,1-10H3,(H2,101,137)(H2,102,138)(H,120,145)(H,121,146)(H,122,153)(H,123,149)(H,124,152)(H,125,154)(H,126,156)(H,127,150)(H,128,143)(H,129,142)(H,130,147)(H,131,148)(H,132,139)(H,133,144)(H,134,151)(H,135,155)(H,140,141)(H,158,159)(H4,103,104,113)(H4,105,106,114)(H4,107,108,115)(H4,109,110,116)(H4,111,112,117)/t50-,51-,52-,57-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,74-,75-,76-,77-/m0/s1

InChI key

ZJCXKXFAOCLSRV-RYLVIXIQSA-N

유전자 정보

human ... LYZ(4069)

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관련 카테고리

일반 설명

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

애플리케이션

Lysozyme from human neutrophils has been used in the checkerboard method for establishing a synergy between peptidomimetic 1 and immune effector molecules against E. coli.

생화학적/생리학적 작용

Lysozymes participates in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

단위 정의

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

물리적 형태

Lyophilized from 50 mM sodium acetate, pH 6.0, with 100 mM NaCl

기타 정보

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

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문서

Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

프로토콜

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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