추천 제품
Quality Level
형태
liquid
사용
sufficient for 100 reactions
sufficient for 400 reactions
특징
Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart
농도
0.75 units/reaction (50 μL reaction volume)
기술
PCR: suitable
색상
red
입력
purified DNA
응용 분야
agriculture
배송 상태
wet ice
저장 온도
−20°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
JumpStart REDTaq ReadyMix PCR Reaction Mix for High Throughput PCR is formulated with REDTaq Genomic DNA polymerase for amplification of more complex templates and genomic templates. This mix contains optimized enzyme and dye concentrations to provide increased length and yield on the more difficult templates.
JumpStart™ REDTaq® ReadyMix™PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The Master mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL of the ReadyMix to primers, template, and water to a final reaction volume of 50 μL. JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.
애플리케이션
JumpStart™REDTaq®ReadyMix™ Reaction Mix has been used:
- For multiplex PCR
- For amplification with reduced non-specific amplification
- For high throughput PCR amplification of complex templates
- In the amplification of primers developed for the pathogenicity-associated genes of Clavibacter michiganensis
- For PCR amplification of the human TMPRSS6 gene
특징 및 장점
- JumpStart™ REDTaq® DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
- JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration
- REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications
- Inert red dye allows for easy verification that reagent has been mixed properly
- Other loading dyes are not required. Aliquots may be directly loaded onto an agarose gel after the reaction
포장
Default reaction volume is 50 μL
20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
800RXN is packaged as 1 X 20 mL
20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
800RXN is packaged as 1 X 20 mL
단위 정의
One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 °C.
기타 정보
View more detailed information on JumpStart REDTaq and ReadyMix products at www.sigma-aldrich.com/hotstart.
법적 정보
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
관련 제품
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Jarred Yasuhara-Bell et al.
International journal of systematic and evolutionary microbiology, 65(Pt 3), 811-826 (2014-12-08)
The genus Clavibacter contains one recognized species, Clavibacter michiganensis. Clavibacter michiganensis is subdivided into subspecies based on host specificity and bacteriological characteristics, with Clavibacter michiganensis subsp. michiganensis causing bacterial canker of tomato. Clavibacter michiganensis subsp. michiganensis is often spread through
Wenqin Wang et al.
BMC plant biology, 10, 205-205 (2010-09-18)
Members of the aquatic monocot family Lemnaceae (commonly called duckweeds) represent the smallest and fastest growing flowering plants. Their highly reduced morphology and infrequent flowering result in a dearth of characters for distinguishing between the nearly 38 species that exhibit
E Beutler et al.
Blood cells, molecules & diseases, 44(1), 16-21 (2009-10-13)
Male subjects with iron deficiency from the general population were examined for polymorphisms or sporadic mutations in TMPRSS6 to identify genetic risk factors for iron deficiency anemia. Three uncommon non-synonymous polymorphisms were identified, G228D, R446W, and V795I (allele frequencies 0.0074
John C Hafner et al.
Journal of biogeography, 38(6), 1077-1097 (2011-08-13)
AIM: The rodent genus Microdipodops (kangaroo mice) includes two sand-obligate endemics of the Great Basin Desert: M. megacephalus and M. pallidus. The dark kangaroo mouse, M. megacephalus, is distributed throughout the Great Basin and our principal aims were to formulate
'Touchdown' PCR to circumvent spurious priming during gene amplification.
R H Don et al.
Nucleic acids research, 19(14), 4008-4008 (1991-07-25)
문서
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
프로토콜
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.
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