콘텐츠로 건너뛰기
Merck
모든 사진(2)

Key Documents

D5809

Sigma-Aldrich

JumpStart AccuTaq LA DNA Polymerase

Hot-start high fidelity Taq enzyme, 10X buffer included

동의어(들):

JumpStart® AccuTaq LA DNA Polymerase

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
41106314
NACRES:
NA.55

Quality Level

형태

liquid

사용

sufficient for 125 reactions
sufficient for 500 reactions

특징

Long & Accurate PCR
dNTPs included: no
hotstart

농도

2.5 unit/μL

기술

PCR: suitable

색상

colorless

입력

purified DNA

적합성

suitable for PCR

배송 상태

wet ice

저장 온도

−20°C

일반 설명

JumpStart AccuTaq LA DNA Polymerase is a combination of Sigma′s high-fidelity blend, AccuTaq long and accurate (LA) DNA Polymerase, and the Taq neutralizing JumpStart Taq antibody. This specially formulated enzyme is the best choice for long-distance and high-fidelity PCR, multiplex PCR, and PCR amplification of targets with variable lengths, such as amplification of cDNA libraries. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot-start mechanism provides increased specificity and higher target yield in comparison to standard amplification.

애플리케이션

JumpStart AccuTaq LA DNA Polymerase has been used:
  • in polymerase chain reaction (PCR) for the amplification of soil DNA samples
  • in accurate long range PCR of histone H2A.Z in bacterial artificial chromosome (BACs)
  • as a component of the PCR reaction mix for amplification of viral DNA through conventional PCR

특징 및 장점

  • JumpStart AccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
  • Up to 6.5× greater fidelity in comparison to Taq DNA polymerase making it the enzyme of choice for multiplex PCR
  • Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA
  • Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods

포장

Supplied with 10× reaction buffer.

단위 정의

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

기타 정보

View more detailed information on Accutaq products at www.sigma-aldrich.com/hotstart.

법적 정보

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
Jacobs is a registered trademark of Jacobs
JumpStart is a trademark of Sigma-Aldrich Co. LLC

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

개인 보호 장비

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Pier-Luc Tremblay et al.
mBio, 4(1), e00406-e00412 (2012-12-28)
It has been predicted that the Rnf complex of Clostridium ljungdahlii is a proton-translocating ferredoxin:NAD(+) oxidoreductase which contributes to ATP synthesis by an H(+)-translocating ATPase under both autotrophic and heterotrophic growth conditions. The recent development of methods for genetic manipulation
Elemental stoichiometry indicates predominant influence of potassium and phosphorus limitation on arbuscular mycorrhizal symbiosis in acidic soil at high altitude
Khan MH, et al.
Journal of Plant Physiology, 189, 105-112 (2015)
Expression of Na+-dependent citrate transport in a strongly metastatic human prostate cancer PC-3M cell line: regulation by voltage-gated Na+ channel activity.
Mycielska, M.E., et al.
The Journal of Physiology, 253.2, 393-393 (2005)
Maja Łebska et al.
The Journal of biological chemistry, 285(9), 6217-6226 (2009-12-19)
Maize eukaryotic translation initiation factor 5A (ZmeIF5A) co-purifies with the catalytic alpha subunit of protein kinase CK2 and is phosphorylated by this enzyme. Phosphorylated ZmeIF5A was also identified after separation of maize leaf proteins by two-dimensional electrophoresis. Multiple sequence alignment
Amber L Mosley et al.
The Journal of biological chemistry, 278(22), 19660-19666 (2003-04-01)
Induction of insulin gene expression in response to high blood glucose levels is essential for maintaining glucose homeostasis. Although several transcription factors including Beta-2, Ribe3b1, and Pdx-1 have been shown to play a role in glucose stimulation of insulin gene

문서

Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

프로토콜

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.