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D8187

Sigma-Aldrich

JumpStart™ REDTaq® DNA Polymerase

Hot-start Taq enzyme with inert dye, 10X buffer included

동의어(들):

Hot start DNA polymerase, Hot start Taq

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About This Item

MDL number:
MFCD00166026
UNSPSC 코드:
12352204
NACRES:
NA.55

Quality Level

200

형태

liquid

사용

sufficient for 250 reactions
 sufficient for 2500 reactions
 sufficient for 50 reactions

특징

dNTPs included: no
hotstart

농도

1 unit/μL

기술

PCR: suitable

색상

red

입력

purified DNA

적합성

suitable for PCR

배송 상태

wet ice

저장 온도

−20°C

일반 설명

JumpStart™ REDTaq® DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.

애플리케이션

JumpStart™ REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) for the amplification of:
  • insulin-enterotoxin ricin fusion gene (INS-RTB)
  • endothelial cells DNA derived from reverse transcribed RNA
  • leg genomic DNA from cricket flies
  • mitochondrial gene by conventional PCR
JumpStart™ REDTaq® DNA Polymerase is also suitable for DNA methylation analysis.

특징 및 장점

  • Reduces non-specific amplification
  • Increased target yield and specificity
  • Higher the amplification irrespective of the target concentration
  • Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
  • Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
  • Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
  • Assembled PCR reactions can be placed at room temperature for up to 2 hours

포장

The enzyme is provided with an optimized 10× reaction buffer.

단위 정의

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

기타 정보

View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.

법적 정보

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

관련 제품

제품 번호
설명
가격

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2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, 10 mM in H2O

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Thymidine 5′-triphosphate sodium salt solution, 10 mM (pH 7.0)

D7045

2′-Deoxycytidine 5′-triphosphate disodium salt, 10 mM, aqueous solution

D8418

Dimethyl sulfoxide, for molecular biology

M8662

Mineral oil, PCR Reagent

D6920

2′-Deoxyadenosine 5′-triphosphate sodium salt solution, 10 mM

B0300

Betaine solution, 5 M, PCR Reagent

W1754

Water, PCR Reagent

D4184

JumpStart™ Taq DNA Polymerase, without MgCl2

P2893

JumpStart™ Taq ReadyMix™, Complete optimized reagent for hot-start PCR at 2X concentration

D7295

Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

D9307

JumpStart™ Taq DNA Polymerase, with MgCl2

P1107

JumpStart™ REDTaq® ReadyMix™ Reaction Mix, for High-throughput PCR of complex templates

픽토그램

Exclamation mark
GHS07

신호어

Warning

유해 및 위험 성명서

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

The field cricket Gryllus assimilis and two new sister species (Orthoptera: Gryllidae).
Weissman, D.B., et al.
Annals of the Entomological Society of America, 102, 367-367 (2009)
Billions and billions sold: pet-feeder crickets (Orthoptera: Gryllidae), commercial cricket farms, an epizootic densovirus, and government regulations make for a potential disaster
Weissman DB, et al.
Zootaxa, 3504(1), 67-88 (2012)
James E Carter et al.
Molecular biotechnology, 44(2), 90-100 (2009-11-10)
Onset of juvenile Type 1 diabetes (T1D) occurs when autoreactive lymphocytes progressively destroy the insulin-producing beta-cells in the pancreatic Islets of Langerhans. The increasing lack of insulin and subsequent onset of hyperglycemia results in increased damage to nerves, blood vessels
Expression of a ricin toxin B subunit: insulin fusion protein in edible plant tissues
Carter JE, et al.
Molecular Biotechnology, 44(2), 90-100 (2010)
Contributions of VEGF to age-dependent transmural gradients in contractile protein expression in ovine carotid arteries
Butler SM, et al.
American Journal of Physiology. Cell Physiology, 301(3), C653-C666 (2011)
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문서

Introduction and Historical Timelines

Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

프로토콜

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Antibody-Enzyme Mediated Hot Start PCR Protocol

When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.

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