MAK451

Glucose-6-Phosphate Dehydrogenase Assay Kit

Sufficient for 100 colorimetric tests

• 동의어(들): G6PD Assay Kit
• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• Quality Level: 100
• 입력: plasma; serum; tissue; cell culture medium
• 검출 방법: colorimetric
• 관련 질환(들): hematological disorder, anemia
• 저장 온도: −20°C

설명

일반 설명

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia.

애플리케이션

The Glucose-6-Phosphate Dehydrogenase Assay Kit may be used for:

• Metabolism Research
• Drug Discovery

특징 및 장점

• Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose-6-phosphate dehydrogenase levels across a wide range from 0.2 to 100 U/L, offering both convenience and sensitivity.
• Compatibility with High-Throughput Systems: Effortlessly incorporate our kit into high-throughput handling systems, guaranteeing seamless integration and maintaining efficiency and accuracy in your laboratory operations.

적합성

The kit is suitable for the quantitative determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism in plasma, serum, tissue and culture media, etc.

원리

The non-radioactive, colorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit is based on the reduction of tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅ nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

기타 정보

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안전성 정보

• 픽토그램: GHS08
• 신호어: Warning
• 유해 및 위험 성명서: H341
• 예방조치 성명서: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class Code: 12 - Non Combustible Liquids