CS0790

Antioxidant Assay Kit

sufficient for 200 tests

• 동의어(들): Oxidative Stress Detection Kit
• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• 사용: sufficient for 200 tests
• Quality Level: 200
• 검출 방법: colorimetric
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Free radicals or reactive oxygen species (ROS) are produced during biochemical redox reactions as part of normal physiological cell metabolism (protection from infectious organisms) and as a response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation. Once formed, ROS attack cellular components causing damage to lipids, proteins, and DNA, which can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.

In the presence of antioxidants the radical cation is suppressed to an extent dependent on the activity of the antioxidant and the color intensity is decreased proportionally. Trolox, a water-soluble vitamin E analogue, serves as a standard or a control antioxidant.

애플리케이션

Antioxidant assay kit provides all of the reagents required for an efficient measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates. It has also been used to measure the radical cation (2,2′-azino-di-[3-ethylbenzthiazoline sulphonate]) (ABTS+) scavenging capacity.

It was tested on A431 and CHO cell lysates; rat brain, liver, and kidney lysates; human plasma, serum, urine, and saliva.

적합성

Suitable for the measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates

원리

The principle of the antioxidant assay is formation of a ferryl myoglobin radical from metmyoglobin and hydrogen peroxide, which oxidizes the ABTS (2,2¢-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) to produce a radical cation, ABTS·+ , a soluble chromogen that is green in color and can be determined spectrophotometrically at 405 nm.

제조 메모

Reagents are to be prepared with ultrapure water.

재구성

Myoglobin Solutions are to be stored at -20C after reconstitution.
Trolox Working Solution is to be stored at -20C after reconstitution.
ABTS Substrate Solution is to be stored at 4C for 2 weeks or at -20C for 1 month.

법적 정보

키트 구성품 전용

• Assay Buffer 10X 30 mL
• Stop Solution 20 mL
• Hydrogen Peroxide, 3% solution 1 mL
• Myoglobin from horse heart 2 x 1
• (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2 x 1
• ABTS, 10 mg tablets 4 tab
• Phosphate-Citrate Buffer, pH 5 4 tab

안전성 정보

• 픽토그램: GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H318,H335,H412
• 예방조치 성명서: P261 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable