MAK452

Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit

Sufficient for 100 colorimetric tests

• 동의어(들): G6PDH Inhibitor Screening Kit
• UNSPSC 코드: 12161503
• NACRES: NA.54

속성

• Quality Level: 100
• 검출 방법: colorimetric
• 관련 질환(들): hematological disorder, anemia; cancer
• 저장 온도: −20°C

설명

일반 설명

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics.

애플리케이션

The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit may be used for:

• Cancer Research
• Drug Discovery

특징 및 장점

• Efficient Inhibitor Screening: Our kit allows for efficient screening of G6PDH inhibitors using just 5 μL of test compound, minimizing effort while ensuring accurate results.
• Compatibility with High-Throughput Systems: Seamlessly integrating into high-throughput handling systems, our kit guarantees efficiency and accuracy, streamlining your screening processes.

적합성

The kit is suitable for high throughput inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.

원리

The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅ nm. The increase in absorbance at A₅₆₅ nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

안전성 정보

• 픽토그램: GHS08
• 신호어: Warning
• 유해 및 위험 성명서: H341
• 예방조치 성명서: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class Code: 12 - Non Combustible Liquids