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Merck
모든 사진(2)

주요 문서

MAK061

Sigma-Aldrich

Isocitrate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.84

사용

sufficient for 100 colorimetric tests

검출 방법

colorimetric

저장 온도

−20°C

일반 설명

The new Isocitrate Assay Kit, MAK524, is now available! Isocitrate is a TCA cycle intermediate that is formed from citrate by the activity of the enzyme aconitase. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. In plants and lower organisms, isocitrate is the branch point from which the glyoxylate shunt operates. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. The Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples.

적합성

Suitable for quantifying isocitrate in a variety of samples including tissue, cells, food and beverages

원리

Isocitrate concentration is determined by a coupled enzyme assay, which results in a colorometric (450 nm) product, proportional to the isocitrate present. Typical sensitivities of detection for this kit are 1-20 nmoles (0.2 - 5 μg) of isocitrate.

픽토그램

Health hazardCorrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

문서

Sigma-Aldrich presents an article about how proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metabolites away from mitochondrial oxidative phosphorylation, many tumor cells exhibit increased mitochondrial activity.

프로토콜

We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.

Chromatograms

application for HPLC

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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