MAK499

Glutamate Dehydrogenase (GDH) Assay Kit

sufficient for 100 colorimetric tests

• 동의어(들): Assay Kit for Glutamate Dehydrogenase Activity
• UNSPSC 코드: 12352204
• NACRES: NA.84

속성

• Quality Level: 100
• 응용 분야: agriculture
• 검출 방법: colorimetric
• 관련 질환(들): cancer; gastrointestinal diseases
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Glutamate dehydrogenase (GLDH) is an enzyme which catalyzes the interconversion of glutamate and α-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the study of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections.

특징 및 장점

• Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
• Compatible with high-throughput handling systems.

적합성

The kit is suitable for glutamate dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue, and culture media.)

원리

The non-radioactive, colorimetric Glutamate Dehydrogenase (GDH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

안전성 정보

• 픽토그램: GHS02,GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H225,H290,H315,H319,H336
• 예방조치 성명서: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2