LYSC9001

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, powder

• 동의어(들): API, Lysyl endopeptidase, Protease I
• 효소 위원회 번호: 3.4.21.50 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Achromobacter lyticus
• Quality Level: 200
• 재조합: expressed in E. coli (206-473aa)
• Grade: Proteomics Grade
• 양식: lyophilized powder
• 최적 pH: 8.5
• pH 범위: 9.0-9.5(catalytic)
• UniProt 수납 번호: P15636
• 저장 온도: −20°C

설명

일반 설명

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

특이성

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

애플리케이션

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

포장

1mg/ml in Plastic

제조 메모

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

재구성

Reconstitute in sterile water.

저장 및 안정성

Store product at -20°C. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles once the protein has been resolubilized in sterile water.

기타 정보

For R&D only.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H315 - H317 - H319 - H334
• 예방조치 성명서: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3