추천 제품
생물학적 소스
Escherichia coli (O55:B5)
Quality Level
무균
γ-irradiated
제품 라인
BioXtra
양식
lyophilized powder
정제법
gel-filtration chromatography
기술
cell culture | mammalian: suitable
불순물
<3% Protein (Lowry)
solubility
H2O: 5 mg/mL, faintly hazy to hazy
저장 온도
2-8°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Lipopolysaccharides (LPS) are complex glycolipids that contain several components. Enterobacteriaceae, such as E. coli, have highly conserved lipid A linked to the antigenic O-polysaccharide. Hydrophobic lipid A forms the outer most component that plays a role in the biological properties attributed to endotoxic LPS. A short core oligosaccharide (core OS) is found to be attached to lipid A. Enterobacteria contain an antigenic, repeat-structure O-polysaccharide (O-PS) attached to the distal core OS to form smooth LPS. O-PS in E. coli contains ∼170 distinct antigens that interact with the host immune system. Based on the core OS structures in E. coli, they have been designated K-12 and R1 to R4.
애플리케이션
LPS (lipopolysaccharide) from Escherichia coli 055:B5 has been used:
- to stimulate human PBMC to secrete cytokine.
- to stimulate wild type embryonic fibroblasts and mutants for the induction of phosphorylation of p56
- in the measurement of tumor necrosis factor-α levels and nuclear factor-κB p65 activities after stimulation
- in the stimulation of hamster lymphocytes in vitro
- to induce activated dendritic morphology in murine macrophage cell line in a study
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
생화학적/생리학적 작용
LPS (lipopolysaccharide) is a major constituent of the outer membrane of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. The LPS confers antigenic and endotoxic characters to the bacteria. They are also important in resistance to infection by phages.
재구성
Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.
기타 정보
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
관련 제품
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 2 Oral
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
이미 열람한 고객
Cheng Yang Ng et al.
Scientific reports, 7(1), 13683-13683 (2017-10-24)
Cytosolic phospholipase A
PURIFICATION AND CHARACTERIZATION OF THE LIPID A COMPONENT OF THE LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI.
A J BURTON et al.
Biochemistry, 3, 411-418 (1964-03-01)
Christopher D Hammerbeck et al.
Journal of virology, 85(19), 9929-9944 (2011-07-22)
Andes virus (ANDV) is associated with a lethal vascular leak syndrome in humans termed hantavirus pulmonary syndrome (HPS). In hamsters, ANDV causes a respiratory distress syndrome closely resembling human HPS. The mechanism for the massive vascular leakage associated with HPS
Natalie M Otto et al.
Critical care (London, England), 12(4), R107-R107 (2008-08-20)
Hyperglycemia is associated with negative outcomes in various settings of critical illness; infectious complications, especially, seem to be increased. On the other hand, intensive insulin therapy (IIT) has been shown to improve outcome in clinical trials. Whether normoglycemia itself or
Mazhar Adli et al.
The Journal of biological chemistry, 281(37), 26976-26984 (2006-07-15)
Nuclear factor kappaB (NF-kappaB) has been studied extensively as an inducible transcriptional regulator of the immune and inflammatory response. NF-kappaB activation downstream of lipopolysaccharide or cytokine stimulation is controlled by the IkappaB kinase complex, which contains IKKalpha and IKKbeta. Significantly
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