L2625

L-Lactic Dehydrogenase from bovine heart

Type III, ammonium sulfate suspension, ≥500 units/mg protein

• 동의어(들): Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD
• CAS Number: 9001-60-9
• 효소 위원회 번호: 1.1.1.27 (BRENDA, IUBMB)
• EC Number: 232-617-8
• MDL number: MFCD00131466
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Type III
• 양식: ammonium sulfate suspension
• 특이 활성도: ≥500 units/mg protein
• UniProt 수납 번호: P19858; Q5E9B1
• 외래 활성: pyruvate kinase ≤0.03%
• 저장 온도: 2-8°C
• 유전자 정보: cow ... LDHA(281274) , LDHB(281275)

설명

일반 설명

Research area: Cell signaling

Lactate dehydrogenase (LDH) is a cytoplasmic enzyme that belongs to the oxidoreductase’s family. It is ubiquitously expressed in body tissues but at high levels in muscle, liver, and kidney. LDH exists as tetramer and comprises of two subunits (H and M). The LDH of eukaryotes undergo active-site loop gating for their catalytic functionality. L-lactate, which is the major enantiomeric form of lactate in vertebrates, is produced by the L-isomers of LDH enzyme encoded by the LDHC gene.

애플리케이션

L-Lactic Dehydrogenase from bovine heart has been used as a standard in cytotoxicity assay. It has also been used in glutamic pyruvic transaminase (GPT) assay.

생화학적/생리학적 작용

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Lactate dehydrogenase (LDH) is a crucial enzyme that plays a vital role in the anaerobic metabolic pathway. It facilitates the conversion of lactate to pyruvate and NAD⁺ to NADH, and vice versa, within cells. This enzymatic reaction plays a crucial role in cellular energy production, particularly in environments with low oxygen levels or hypoxic conditions. LDH activity is also significant in tumor glycolysis and the Warburg effect. LDH is released into the bloodstream when the cells are damaged or undergo death. Therefore, LDH can be used as a potential marker of cell injury and death. High lactate dehydrogenase activity is associated with various medical conditions, such as liver disease, anemia, heart attack, bone fractures, muscle trauma, and certain types of cancer.

단위 정의

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

물리적 형태

Crystalline suspension in 2.1 M (NH₄)₂SO₄ solution, pH 6.0

분석 메모

Protein determined by biuret

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)