L3916

L-Lactic Dehydrogenase from bovine heart

1000 units/mL

• 동의어(들): Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD
• CAS Number: 9001-60-9
• 효소 위원회 번호: 1.1.1.27 (BRENDA, IUBMB)
• EC Number: 232-617-8
• MDL number: MFCD00131466
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 형태: solution
• Quality Level: 200
• 특이 활성도: 1000 units/mL
• 저장 온도: 2-8°C

설명

일반 설명

LDH (lactic dehydrogenase) is a glycolytic enzyme. It is found in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It comprises of five isoenzyme forms. LDH possess a tetrameric structure.

애플리케이션

L-Lactic Dehydrogenase from bovine heart has been used:

• as a reference standard to measure the LDH (lactic dehydrogenase)
• to quench protein-bound NADH in cells
• to measure the lactate released by the cells in suspension

For use in enzymatic determination of lactate or pyruvate.

생화학적/생리학적 작용

LDH (lactic dehydrogenase) helps in the reversible conversion of lactate to pyruvate.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

단위 정의

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

물리적 형태

Suspension in 2.2 M ammonium sulfate

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)