59747

Lactic Dehydrogenase, recombinant from E. coli

≥90 U/mg

• 동의어(들): (S)-Lactate: NAD⁺ oxidoreductase, L-Lactate Dehydrogenase, Lactate
• CAS Number: 9001-60-9
• 효소 위원회 번호: 1.1.1.27 (BRENDA, IUBMB)
• EC Number: 232-617-8
• MDL number: MFCD00131466
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 양식: powder
• 특이 활성도: ≥90 U/mg
• 저장 온도: −20°C

설명

일반 설명

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.

애플리케이션

Lactic Dehydrogenase, recombinant from E. coli has been used:

• in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
• to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
• in in vitro DltC D-alanylation assay

생화학적/생리학적 작용

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.

Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.

단위 정의

One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)