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Merck
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Key Documents

I5513

Sigma-Aldrich

Imidazole

for molecular biology, ≥99% (titration)

동의어(들):

3-Azapyrrole, 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline

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About This Item

실험식(Hill 표기법):
C3H4N2
CAS Number:
Molecular Weight:
68.08
Beilstein:
103853
EC Number:
MDL number:
UNSPSC 코드:
12352005
eCl@ss:
39161001
PubChem Substance ID:
NACRES:
NA.25

Grade

for molecular biology

Quality Level

vapor pressure

<1 mmHg ( 20 °C)

분석

≥99% (titration)

형태

crystalline powder

저장 조건

dry at room temperature

기술

electrophoretic mobility shift assay: suitable
molecular cloning: suitable
protein purification: suitable

색상

white

pH

6.2-7.8 (25 °C)

pKa(25 °C)

6.95

bp

256 °C (lit.)

mp

88-91 °C (lit.)

solubility

water: 500 mg/mL, clear, colorless to very faintly yellow

density

1.004 g/cm3 at 20—25 °C
1.006 g/cm3 at 20—25 °C

적합성

suitable for molecular biology

응용 분야

diagnostic assay manufacturing
genomic analysis
life science and biopharma
microbiology

외래 활성

DNase, RNase and protease, none detected

저장 온도

room temp

SMILES string

c1c[nH]cn1

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Imidazole, also known as Glyoxalin, is an aromatic heterocyclic diazole and a key biological buffer with a pH range of 6.2 to 7.8 at 25°C. It has a wide range of applications in molecular biology, biochemistry, and proteomics. In proteomics, imidazole is essential for eluting histidine-tagged proteins from IMAC columns. It is a strong chelator of divalent cations, such as nickel and zinc, which disrupts the interaction between the His-tag and the metal ions. This allows for the efficient elution of purified proteins.

Furthermore, imidazole is indispensable for enhancing protein purity. It is widely used in preparing buffer solutions optimized for downstream purification columns. Imidazole solutions are also effective elution and washing agents throughout protein purification processes. Additionally, imidazole can be used for assays of horseradish peroxidase (HRP) and reverse staining of SDS-PAGE gels for protein detection. Imidazole is a versatile and valuable reagent for a wide range of biological applications. It is an essential component of many buffers and is also used in protein purification and detection protocols.

애플리케이션

Imidazole has been used

  • in the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein
  • in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography
  • as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells
  • as a binding buffer, wash buffer, and elution buffer in a study involving protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture
Excellent for buffers in the range of pH 6.2-7.8

생화학적/생리학적 작용

Imidazole derivatives have antibacterial, antifungal and anticancer functionality. It interacts with DNA and also binds to protein and stops cell division. It also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes. Imidazole based anticancer drug find applications in cancer chemotherapy. It is used as buffer component for purification of the histidine tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC).

특징 및 장점

  • Suitable for Molecular Biology and Biochemical Research
  • Can be used as a Buffer component, for Protein separation
  • Free from DNase, NICKase, RNase, and Protease
  • Effective Buffering from pH 6.2 to 7.8 (25 °C) with a pKa of 6.9 (25 °C)

기타 정보

For additional information on our range of Biochemicals, please complete this form.

픽토그램

Health hazardCorrosionExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point (°F)

293.0 °F - closed cup

Flash Point (°C)

145 °C - closed cup

개인 보호 장비

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Imidazole: Having versatile biological activities
Verma A, et al.
Journal of Chemistry, 2013 (2013)
Functional expression of novel human and murine AKR1B genes
Salabei JK, et al.
Chemico-Biological Interactions, 191(1-3), 177-184 (2011)
Imidazoles as potential anticancer agents
Ali I, et al.
MedChemComm, 8(9), 1742-1773 (2017)
Isolation of Phagosomes from Dendritic Cells by Using Magnetic Beads
Manoury B
Bio-protocol, 3(14), 177-184 (2013)
[Purification of proteins using polyhistidine affinity tags
Bornhorst JA and Falke JJ
Methods in Enzymology, 326, 245-254 (2000)

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