56749

Imidazole

BioUltra, ≥99.5% (GC)

• 동의어(들): 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline
• 실험식(Hill 표기법): C₃H₄N₂
• CAS Number: 288-32-4
• Molecular Weight: 68.08
• Beilstein: 103853
• EC Number: 206-019-2
• MDL number: MFCD00005183
• UNSPSC 코드: 12352005
• eCl@ss: 39161001
• PubChem Substance ID: 57651597
• NACRES: NA.25

속성

• vapor pressure: <1 mmHg ( 20 °C)
• Quality Level: 200
• 제품 라인: BioUltra
• 분석: ≥99.5% (GC)
• 형태: powder or crystals
• 불순물: insoluble matter, passes filter test; ≤0.2% water
• 무기 잔류물(900 °C): ≤0.05%
• pH: 9.5-11.0 (25 °C, 0.1 M in H₂O)
• pKa(25 °C): 6.95
• bp: 256 °C (lit.)
• mp: 88-91 °C (lit.)
• solubility: H₂O: 0.1 M at 20 °C, clear, colorless
• 음이온 미량물: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• 양이온 미량물: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• UV 흡수: λ: 260 nm A_max: 0.10; λ: 280 nm A_max: 0.10
• SMILES string: c1c[nH]cn1
• InChI: 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
• InChI key: RAXXELZNTBOGNW-UHFFFAOYSA-N

설명

애플리케이션

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.

Excellent for buffers in the range of pH 6.2-7.8

기타 정보

Buffer for assay of horseradish peroxidase

안전성 정보

• 픽토그램: GHS05,GHS07,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H302 - H314 - H360D
• 예방조치 성명서: P260 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• Flash Point (°F): 293.0 °F - closed cup
• Flash Point (°C): 145 °C - closed cup
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges