I2276

Interleukin-12 human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• 동의어(들): IL-12
• MDL number: MFCD00212975
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in baculovirus infected Sf21 cells
• 분석: ≥97% (SDS-PAGE and N-terminal analysis)
• 양식: lyophilized powder
• 효능: 0.01-0.05 ng/mL ED₅₀
• 분자량: glycoprotein 75 kDa
• 포장: pkg of 5 μg
• 기술: cell culture | mammalian: suitable
• 불순물: endotoxin, tested
• UniProt 수납 번호: P29459,; P29460
• 저장 온도: −20°C
• 유전자 정보: human ... IL12A(3592) , IL12B(3593)

설명

생화학적/생리학적 작용

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)