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Merck
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주요 문서

GE28-9031-33

Protein A High Performance MultiTrap

Cytiva 28-9031-33

동의어(들):

High Performance Protein A, MultiTrap Protein A

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About This Item

UNSPSC 코드:
41105323
NACRES:
NA.25

ligand

native protein A

material

polypropylene and polyethylene

특징

wetted part: no

포장

pkg of 4 ea

제조업체/상표

Cytiva 28-9031-33

저장 조건

(20% Ethanol)

파라미터

30 °C max. temp.
4 °C min. temp.

크기

127.8 mm × 85.5 mm × 30.6 mm

Matrix

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning

2-10

작동 범위

3-9

저장 온도

2-8°C

관련 카테고리

일반 설명

Protein A Sepharose High Performance has high affinity for the Fc region of IgG from a variety of species.

Protein A Sepharose High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

특징 및 장점

  • Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
  • Flexible format, with protocols for classic elution and cross-link method.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Easy scale-up with HiTrap® Protein A HP prepacked columns.
  • Prepacked with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses.
  • MultiTrap 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

HiTrap is a registered trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

문서

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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