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주요 문서

GE17-5111-01

HiTrap® IGY Purification

Cytiva 17-5111-01, pack of 1 × 5 mL

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About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

ligand

2-mercaptopyridine

포장

pack of 1 × 5 mL

제조업체/상표

Cytiva 17-5111-01

저장 조건

(20% Ethanol)

파라미터

<20 mL/min flow rate
42 psi

베드 크기

16 mm × 25 mm

베드 용적

5 mL

컬럼 I.D.

16 mm

Matrix

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning

2-13

작동 범위

3-11

저장 온도

2-30°C

관련 카테고리

일반 설명

HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose High Performance for fast and easy purification of IgY from egg yolk.

애플리케이션

HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

특징 및 장점

  • Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
  • Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose High Performance.
  • Recovery and purity of IgY are excellent.
  • Can be operated with a liquid chromatography system such as ÄKTA® design.
  • The top and bottom frits are manufactured from porous polyethylene
  • Connectors for usage with different chromatography systems and other equipment are included

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


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시험 성적서(COA)

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프로토콜

This page shows how to prepare samples for purification with affinity chromatography.

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