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주요 문서

GE17-1408-01

HiTrap® Desalting Columns

Cytiva 17-1408-01, pack of 5 × 5 mL

동의어(들):

Purification Columns

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About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

포장

pack of 5 × 5 mL

제조업체/상표

Cytiva 17-1408-01

저장 조건

dry media

파라미터

42 psi

베드 크기

16 mm × 25 mm

베드 용적

5 mL

컬럼 I.D.

16 mm

Matrix

cross-linked dextran

입자 크기

>20 μm (dry)

cleaning

2-13

작동 범위

2-13

적합성

suitable for bioprocess medium

일반 설명

HiTrap® Desalting is a ready-to-use 5 mL column, prepacked with Sephadex® G-25 Superfine, for fast and convenient desalting and buffer exchange.

HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.

애플리케이션

Desalting and buffer exchange with Sephadex® G-25.

특징 및 장점

  • Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
  • Replaces dialysis as the method of choice for desalting.
  • Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

HiTrap is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

문서

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

프로토콜

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page shows how to prepare samples for purification with affinity chromatography.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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