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주요 문서

GE17-0413-01

HiTrap® Blue High Performance

Cytiva 17-0413-01, pack of 1 × 5 mL

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About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

ligand

Cibacron Blue F3G-A

포장

pack of 1 × 5 mL

제조업체/상표

Cytiva 17-0413-01

파라미터

42 psi

베드 크기

16 mm × 25 mm

베드 용적

5 mL

컬럼 I.D.

16 mm

크기

평균 부품 크기

34 μm

cleaning

3-13

작동 범위

4-12

일반 설명

HiTrap® Blue HP is prepacked with Blue Sepharose High Performance for capture of a wide range of biomolecules, including: albumin, interferon, a broad range of nucleotide-dependent enzymes, α2-macroglobulin, coagulation factors, and related proteins.

애플리케이션

Blue Sepharose High Performance is a group specific adsorbent with affinity for a wide variety of enzymes. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the matrix via the triazine part of the dye molecule. Some Proteins interact biospecifically with the dye due to its structural similarity with nucleotide cofactors, while others, such as albumin and interferon, bind in a less specific manner by electrostatic and/or hydrophobic interactions with the aromatic anionic ligand.

특징 및 장점

  • Can be used for depletion of albumin from serum.
  • Cibacron Blue F3G-A, the ligand, is covalently attached to the highly cross-linked Agarose medium and is stable over a pH range of 4 to 12 for use and 3 to 13 for cleaning.
  • The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC System.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

도움이 필요하시면 연락하세요. 고객 지원 부서

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

프로토콜

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

This page shows how to remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from GE Healthcare.

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

Performing a Purification of NAD+-dependent dehydrogenases and ATP-dependent kinases with 5’ AMP Sepharose 4B, HiTrap Blue HP and Blue Sepharose 6 Fast Flow

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