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Merck
모든 사진(2)

Key Documents

A1271

Sigma-Aldrich

Adenosine 5′-monophosphate–Agarose

lyophilized powder

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About This Item

MDL number:
UNSPSC 코드:
41106500
eCl@ss:
32160414
PubChem Substance ID:
NACRES:
NA.56

생물학적 소스

plant (Sea weed)

Quality Level

형태

lyophilized powder

라벨링 범위

1-5 μmol per mL

기질

cross-linked 4% beaded agarose

기질 활성

cyanogen bromide

기질 부착

C-8

기질 스페이서

9 atoms

저장 온도

−20°C

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관련 카테고리

애플리케이션

Adenosine 5′-monophosphate Agarose (5′-AMP agarose) has been used in affinity chromatography to isolate β and gamma glutamate decarboxylase, which is important for controlling gamma-aminobutyric acid (GABA) synthesis in brain.

물리적 형태

Lyophilized powder stabilized with lactose

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서 라이브러리 방문

F James et al.
The Journal of biological chemistry, 270(38), 22344-22350 (1995-09-22)
The plant enzyme S-adenosylmethionine:methionine S-methyltransferase (EC 2.1.1.12, MMT) catalyzes the synthesis of S-methylmethionine. MMT was purified 620-fold to apparent homogeneity from leaves of Wollastonia biflora. The four-step purification included fractionation with polyethylene glycol, affinity chromatography on adenosine-agarose, anion exchange chromatography
C D Murphy et al.
Applied and environmental microbiology, 67(10), 4919-4921 (2001-09-26)
Streptomyces cattleya is unusual in that it produces fluoroacetate and 4-fluorothreonine as secondary metabolites. We now report the isolation of an NAD(+)-dependent fluoroacetaldehyde dehydrogenase from S. cattleya that mediates the oxidation of fluoroacetaldehyde to fluoroacetate. This is the first enzyme
S J Wu et al.
Journal of neurochemistry, 42(6), 1607-1612 (1984-06-01)
The interactions of two forms of porcine brain glutamate decarboxylase (beta-GAD and gamma-GAD) with the effector ATP were studied by affinity chromatography. A third form, alpha-GAD, was only slightly retarded by the affinity matrix and was eluted in the buffer
M Kato et al.
Plant physiology, 120(2), 579-586 (1999-06-11)
Caffeine synthase (CS), the S-adenosylmethionine-dependent N-methyltransferase involved in the last two steps of caffeine biosynthesis, was extracted from young tea (Camellia sinensis) leaves; the CS was purified 520-fold to apparent homogeneity and a final specific activity of 5.7 nkat mg-1
D L Martin et al.
Journal of neurochemistry, 55(2), 524-532 (1990-08-01)
A major regulatory feature of brain glutamate decarboxylase (GAD) is a cyclic reaction that controls the relative amounts of holoenzyme and apoenzyme [active and inactive GAD with and without bound pyridoxal 5'-phosphate (pyridoxal-P, the cofactor), respectively]. Previous studies have indicated

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