ECM001

Human Fibronectin

recombinant, expressed in HEK 293 cells, powder, suitable for cell culture

• 동의어(들): Fibronectin, CIG, Cold-insoluble globulin, FN
• MDL number: MFCD00131062
• UNSPSC 코드: 12352202
• NACRES: NA.75

속성

• product name: Fibronectin human, recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in HEK 293 cells
• 무균: sterile
• 분석: ≥95% (SDS-PAGE)
• 형태: lyophilized powder
• 분자량: 259.5 kDa (The protein migrates as a higher band on SDS-PAGE due to glycosylation.)
• 포장: pkg of 200 μg
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/μg endotoxin, tested
• solubility: water: soluble
• NCBI 수납 번호: NM_212482.4
• UniProt 수납 번호: P02751
• 배송 상태: ambient
• 저장 온도: −20°C
• 유전자 정보: human ... FN1(2335)

설명

일반 설명

Fibronectin (FN) is a glycoprotein with 5-9% carbohydrates present as N- and O-linked oligosaccharides. It belongs to the ligand glycoprotein family. The FN gene is localized to human chromosome 2q35. FN exists as two major isoforms, plasma-based soluble and extracellular matrix (ECM)-based insoluble form. Fibronectin structure comprises repeats of FNI, FNII and FNIII repeats. It also has fibrin-binding sites and exists as a dimer. Alternative splicing of the FN1 gene leads to the generation of many isoforms. This fibronectin protein is manufactured in human cells using an all-human production system with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.

애플리케이션

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells.
Recommended for use as a cell culture substratum at 1-5 μg/cm² or 0.5-50 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.

Fibronectin human has been used for coating cell culture plates for culturing endothelial progenitor cells. It has also been used as a component of Dulbecco′s modified Eagle′s medium (DMEM) in the in vitro matrigel invasion assay of glioblastoma cells.

생화학적/생리학적 작용

Fibronectin (FN) is essential for embryonic development and mediates cell adhesion and migration functions. It mediates apoptosis in nasopharyngeal carcinoma (NPC). The plasma FN regulates thrombosis. Fibronectin functions as a ligand for the integrin receptor family and contains collagen, glycoprotein and glycosaminoglycan binding sites. Mutation in the FNI gene leads to an imbalance in the soluble and insoluble forms of fibronectin leading to glomerular deposits in hereditary kidney disease, glomerulopathy. Missense mutations in the FN1 gene are implicated in the skeletal dysplasias disorder, Spondylometaphyseal dysplasias.

특징 및 장점

• Human-derived fibronectin
• Produced through recombinant expression in HEK 293 cells
• Low endotoxin levels
• Absence of bacterial contamination

물리적 형태

Supplied as a powder, lyophilized from CAPS buffered saline.

분석 메모

The biological activity of recombinant human fibronectin was tested in culture by measuring the ability of immobilized fibronectin to support adhesion of CHO cells.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable