S5171

Human Fibronectin

formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture

• 동의어(들): sFN
• MDL number: MFCD00284706
• UNSPSC 코드: 12352202
• NACRES: NA.75

속성

• product name: Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in E. coli
• 제품 라인: BioReagent
• 형태: solution
• 분자량: ~235-270 kDa
• 포장: pkg of 5 mg
• 기술: cell culture | mammalian: suitable
• 표면 범위: 1 μg/cm²
• UniProt 수납 번호: P02751
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 유전자 정보: human ... FN1(2335)

설명

애플리케이션

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

성분

Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.

주의사항

Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.

제조 메모

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable