L2020

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane

1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture

• 동의어(들): Laminin
• CAS Number: 114956-81-9
• MDL number: MFCD00081739
• UNSPSC 코드: 12352202
• NACRES: NA.75

속성

• 생물학적 소스: mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
• Quality Level: 300
• 제품 라인: BioReagent
• 형태: aqueous solution
• 분자량: A subunit 400 kDa; B1 subunit 210 kDa; B2 subunit 200 kDa
• 포장: pkg of 1 mg
• 농도: 1-2 mg/mL in Tris-buffered saline
• 기술: cell culture | mammalian: suitable
• 표면 범위: 1‑2 μg/cm²
• 불순물: Microbial Contamination, passes test
• NCBI 수납 번호: NM_008480.2
• UniProt 수납 번호: P02468; P02469; P19137; Q61092
• 결합 특이성: Peptide Source: Collagen
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: mouse ... Lama1(16772) , Lamb1(16777) , Lamc1(226519) , Lamc2(16782)

설명

일반 설명

Laminins are non-collagenous adhesive glycoproteins with a substantial molecular weight. They are heterotrimeric proteins with three disulfide-linked polypeptides known as the α, β, and γ chains. The multiple polypeptide domains of laminins can bind to multiple cell surface receptors, including several integrin dimers, dystroglycan and syndecans, and other ECM components. This extracellular matrix multidomain trimeric glycoprotein is the main component of basal lamina that supports adhesion, proliferation and differentiation.

애플리케이션

Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in:

• insulin-producing cell (IPC) differentiation.
• Infection inhibition assays.
• Proliferation Assay.

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm². The optimal concentration does depend on cell type as well as the application or research objectives.

생화학적/생리학적 작용

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

성분

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.

주의사항

It is recommended to store this product in working aliquots at -20°C.

제조 메모

This product is supplied at a concentration of 1 − 2 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)