F4759

Fibronectin bovine plasma

powder, BioReagent, suitable for cell culture

• 동의어(들): Fibronectin
• EC Number: 289-149-2
• MDL number: MFCD00131062
• UNSPSC 코드: 12352202
• eCl@ss: 42020141
• NACRES: NA.75

속성

• 생물학적 소스: bovine plasma
• Quality Level: 200
• 무균: sterile
• 제품 라인: BioReagent
• 형태: powder
• 분자량: ~450 kDa
• 포장: pkg of 1 mg; pkg of 2 mg; pkg of 5 mg
• 농도: ≥1 mg protein/mL
• 기술: cell culture | mammalian: suitable
• 표면 범위: 1‑5 μg/cm²
• solubility: H₂O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
• NCBI 수납 번호: NM_001163778.1
• UniProt 수납 번호: P07589
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: bovine ... FN1(2807940)

설명

일반 설명

Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.

Fibronectins are adhesive glycoproteins and are found both as cell surface proteins and in plasma. Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

애플리케이션

Fibronectin from bovine plasma is used for the following applications:

• Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
• Cell movement assays (culture plates were coated with fibronectin)
• Cell spreading and immunofluorescence
• Protein specificity
• Integration of sensor films into microfluidic chips
• Histological and Immunohistochemical (IHC) analyses
• Used in primary cell cultures from patients with lung carcinoma

생화학적/생리학적 작용

Fibronectin is a regulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.Study shows that human dental pulp (HDP) cells rely on fibronectin (FN) for initial attachment and subsequent spreading. FN may exhibit mitogenic activity, promoting cell proliferation, and also play a role in regulating the differentiation process of pulp cells into odontoblasts. The presence of FN appears to influence the fate and development of HDP cells, specifically in their differentiation into odontoblasts, which are responsible for dentin formation in teeth.

주의사항

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)