F2518

Human Fibronectin

from human foreskin fibroblasts, powder, suitable for cell culture

• 동의어(들): Fibronectin
• EC Number: 289-149-2
• MDL number: MFCD00131062
• UNSPSC 코드: 12352202
• NACRES: NA.75

속성

• product name: Fibronectin human foreskin fibroblasts, lyophilized powder, suitable for cell culture
• 생물학적 소스: human foreskin fibroblasts
• Quality Level: 200
• 제품 라인: BioReagent
• 형태: lyophilized powder
• 분자량: ~550 kDa
• 포장: pkg of 0.5 mg
• 기술: cell culture | mammalian: suitable
• 표면 범위: 1‑5 μg/cm²
• 불순물: Human Source, none detected (HCV); Human Source, none detected (HIV); Human Source, none detected (HTLV); Human Source, none detected (HbSAg); Microbial Contamination, passes test
• solubility: H₂O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
• NCBI 수납 번호: NM_212482.4
• UniProt 수납 번호: P02751
• 결합 특이성: Peptide Source: Collagen
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... FN1(2335)

설명

일반 설명

Fibronectin 1 (FN1) is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Fibronectin is mostly synthesized by fibroblasts and endothelial cells.It is an adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous and form functional units arranged linearly ontwo subunit arms.

애플리케이션

Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells

Fibronectin human foreskin fibroblasts has been used in extracellular matrix binding assay and probiglycan and cleavage assays. It has also been used in ectoderm (neuronal) differentiation and in the preparation of a semi-solid growth medium.

생화학적/생리학적 작용

Fibronectin 1 (FN1) participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Fibronectin production by normal human foreskin fibroblasts has been shown to improve two-fold after glucocorticoid treatment.

특징 및 장점

• Fibronectin is isolated from human foreskin fibroblasts maintained in serum-free medium.
• It can be used as a cell attachment factor for various cell types, including epithelial, mesenchymal, neuronal, neural crest, endothelial cells, and fibroblasts.
• The starting material is tested and found negative for HIV and HBsAg.
• The product is soluble in sterile water, yielding a clear, colorless solution.
• It can be stored at -20°C for at least three years.

주의사항

Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.

물리적 형태

Lyophilized in CAPS buffered saline

제조 메모

dialyzed against chloroform

재구성

Store reconstituted solution in working aliquots at -20°C or lower.

분석 메모

Protein homogeneity is evaluated by immunoelectrophoresis.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)