E5134

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

• 동의어(들): Sequestrene Na₂, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂
• 실험식(Hill 표기법): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• CAS Number: 6381-92-6
• Molecular Weight: 372.24
• Beilstein: 3900609
• EC Number: 205-358-3
• UNSPSC 코드: 12352107
• PubChem Substance ID: 24894605
• NACRES: NA.31

속성

• Grade: for molecular biology
• Quality Level: 300
• 분석: 99.0-101.0% (titration)
• 형태: powder
• 반응 적합성: reagent type: chelator
• 기술: electrophoresis: suitable
• 불순물: ≤0.005% Insolubles; ≤0.1% Nitrilotriacetic acid (NTA)
• mp: 248 °C (dec.) (lit.)
• solubility: 3 M NaOH: 1.6 g/10 mL
• 양이온 미량물: Fe: ≤0.005%; Pb: ≤0.002%
• 적합성: suitable for electrophoresis; suitable for molecular biology
• 외래 활성: DNase, RNase, NICKase and protease, none detected
• 저장 온도: room temp
• SMILES string: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• InChI key: OVBJJZOQPCKUOR-UHFFFAOYSA-L

설명

일반 설명

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests

애플리케이션

EDTA disodium salt has been used in research:

• as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
• an anticoagulant for whole blood collection
• a stabilizer solution for freezing cell culture samples
• in combination with PBS and trypsin, to trypsinize cells
• for phytoextraction of heavy metals in polluted soils

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

특징 및 장점

• Molecular Biology grade, suitable for many molecular biology applications
• Free of endonucleases DNase, RNase, Nickase, and Protease

기타 정보

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안전성 정보

• 픽토그램: GHS07,GHS08
• 신호어: Warning
• 유해 및 위험 성명서: H332 - H373
• 예방조치 성명서: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• 표적 기관: Respiratory Tract
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)