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Merck
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문서

E5134

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)

동의어(들):

Sequestrene Na₂, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

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모든 사진(6)

About This Item

실험식(Hill 표기법):
C10H14N2Na2O8 · 2H2O
CAS Number:
6381-92-6
Molecular Weight:
372.24
Beilstein:
3900609
EC Number:
205-358-3
UNSPSC 코드:
12352107
PubChem Substance ID:
24894605
NACRES:
NA.31

Grade

for molecular biology

분석

99.0-101.0% (titration)

형태

powder

반응 적합성

reagent type: chelator

기술

electrophoresis: suitable

불순물

≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)

mp

248 °C (dec.) (lit.)

solubility

3  M NaOH: 1.6 g/10 mL

양이온 미량물

Fe: ≤0.005%
Pb: ≤0.002%

적합성

suitable for electrophoresis
suitable for molecular biology

외래 활성

DNase, RNase, NICKase and protease, none detected

저장 온도

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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관련 카테고리

일반 설명

EDTA disodium salt dehydrate chelates metal ions, forming an octahedral complex with divalent cations. This is useful in molecular biology research to inhibit enzymes dependent on activation by metal ions. In diagnostic assays, it acts as an anticoagulant during blood collection for hematological tests

애플리케이션

EDTA disodium salt has been used in research:
  • as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more
  • an anticoagulant for whole blood collection
  • a stabilizer solution for freezing cell culture samples
  • in combination with PBS and trypsin, to trypsinize cells
  • for phytoextraction of heavy metals in polluted soils
Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

특징 및 장점

  • Molecular Biology grade, suitable for many molecular biology applications
  • Free of endonucleases DNase, RNase, Nickase, and Protease

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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픽토그램

Exclamation markHealth hazard
GHS07,GHS08

신호어

Warning

유해 및 위험 성명서

H332 - H373

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

표적 기관

Respiratory Tract

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

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M?ller J, et al.
Clinical Chemistry, 45(9), 1536-1542 (1999)
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