HF1

94101908, rat liver, Epithelial

• UNSPSC 코드: 41106514

속성

• 제품명: HF1, 94101908
• 생물학적 소스: rat liver
• 성장 모드: Adherent
• 핵형: Modal no. 100
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat hepatoma Hybrid [H5xFao]

세포주 설명

HF1 is a clone of hybrid cells resulting from the fusion of H5 and Fao. Expression of the majority of hepatic functions are extinguished. The cells contain a number of chromosomes corresponding to the sum of the parental numbers. HF1 variant which lacks the complete set of tissue-specific functions, appears to be a good candidate for regulatory mutants which control the orderly temporal expression of tissue specific functions during development.

애플리케이션

Analysis of gene expression by molecular techniques.

배양 배지

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 9x1,000 to 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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