CHO Cell Line from Chinese hamster ovary

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85050302, hamster ovary, Epithelial

• 동의어(들): CHO Cells, CHO-ori Cells, Chinese Hamster Ovary Cells, Chinese hamster ovary cell line
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: hamster ovary
• 포장: tube of 5 μg 85050302-DNA-5UG; pkg of vial of cells 85050302-1VL
• 재고 정보: not available in Japan
• 성장 모드: Adherent
• 핵형: Hypodiploid, modal no. 20
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Hamster Chinese ovary

세포주 설명

A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.

애플리케이션

CHO Cell Line from Chinese hamster ovary has been used:

• to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
• to carry out the vaginolysin (VLY) cytolytic activity assay
• as host cells to produce glucagon-like peptide 1 receptor (GLP1R)

Nutritional and gene expression studies

배양 배지

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.