CHO-K1 Cell Line from hamster

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85051005, hamster ovary, Epithelial

• 동의어(들): CHO cell clone K1 Cells, CHOK1 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: hamster ovary
• 포장: tube of 5 μg 85051005-DNA-5UG; pkg of vial of cells 85051005-1VL
• 성장 모드: Adherent
• 핵형: Hypodiploid, modal no. 20
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Hamster Chinese ovary

세포주 설명

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.

애플리케이션

CHO-K1 cell line from hamster has been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.

Nutritional and gene expression studies

배양 배지

Ham′s F12+ 2mM Glutamine+ 10% Foetal Bovine Serum FBS / FCS.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25%Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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