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Merck
모든 사진(3)

Key Documents

B2518

Sigma-Aldrich

Albumin, Acetylated from bovine serum

lyophilized powder, suitable for microbiology

동의어(들):

Ac-BSA, Acetyl BSA, Acetylated BSA

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.31

생물학적 소스

bovine

Quality Level

형태

lyophilized powder

정제법

heat shock fractionation

기원

USA origin

기술

Southern blotting: suitable
blocking: suitable
cell culture | mammalian: suitable

불순물

HIV I and HIVII, HCV and HBsAg, tested negative

pH

7

적합성

suitable for microbiology

UniProt 수납 번호

외래 활성

DNase free
DNase, RNase, NICKase, protease, none detected
NICKase free
Protease free
RNase free

저장 온도

2-8°C

유전자 정보

bovine ... ALB(280717)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Native bovine serum albumin (BSA) is a 66 kDa protein, which belongs to the family of serum albumin. It consists of three domains (I, II and III) and is the major protein found in circulatory system. Native BSA is an α-helical, globular and non-glycosylated protein, with 17-disulfide bonds.

Acetylation of bovine serum albumin modifies various residues, most notably lysine (Lys), and also serine (Ser) and threonine (Thr) to lesser extents. MALDI-TOF mass spectrometry studies on acetylated BSA have indicated a MW value about 3000 Da higher compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. Acetylation of BSA also modifies parallel amino acid residues in any trace contaminating nucleases in the BSA, which inactivates that trace nuclease content. Thus acetylated BSA can be used in molecular biology applications where trace nuclease activity must be minimized.

애플리케이션

Acetylated bovine serum albumin has been used:
  • as a component of protease buffer for protease inhibition assay
  • as a blocking agent in the preparation of cDNA glass array
  • to select hybridoma antibodies sensitive to M-ficolin epitopes by immunofluorometric assay
This B2518 acetylated BSA product has been cited in various publications and protocols, such as the following:






* As an electrophoresis standard: Lindberg, T. et al., J. Pediatr. Gastroenterol. Nutr., 27(1), 30-36 (1998)




* In in situ end-labeling buffer for labeling of DNA fragments: Save, V. et al., ”Detecting and Quantifying Apoptosis in Tissue Sections”, in Apoptosis Methods and Protocols (H.J.M. Brady, ed.). Methods Mol. Biol., Vol. 282, pp. 67-84 (2004)




* In EMSA studies on nucleocytoplasmic shuttling: Ghosh, C.C. et al., “Analysis of Nucleocytoplasmic Shuttling of NF?B Proteins in Human Leukocytes”, in Membrane Trafficking (A. Vancura, ed.). Methods Mol. Biol., Vol. 457, pp. 279-292 (2008)




* In selection buffer for ribosome display studies: Larman, H.B. et al., Nat. Protoc., 9(1), 90-103 (2014)
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.

생화학적/생리학적 작용

Native bovine serum albumin (BSA) acts as a transporter of drugs, hormones and fatty acids. It has calcium binding sites and regulates metal homeostasis.

특징 및 장점

  • Acetylated to inactivate trace nucleases
  • DNase free / Exonuclease free
  • Nickase free / Endonuclease free
  • RNase free
  • Protease free

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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