A9253

L-Amino Acid Oxidase from Crotalus adamanteus

Type I (dried venom)

• 동의어(들): L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)
• CAS Number: 9000-89-9
• 효소 위원회 번호: 1.4.3.2 (BRENDA, IUBMB)
• EC Number: 232-564-0
• MDL number: MFCD00131454
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Crotalus adamanteus
• Quality Level: 200
• 유형: Type I (dried venom); Type I
• 양식: powder
• 분자량: ~130 kDa
• solubility: H₂O: soluble 1.0 mg/mL, clear(lit.)
• 저장 온도: −20°C

설명

일반 설명

L-Amino acid oxidase (LAAO) is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70 kDa. Each molecule of holoenzyme has two flavin adenine dinucleotide (FAD) molecules. LAAO is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. optimum pH is approximately 7.5. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney. In the N-terminal region, it has a βαβ domain with glutamic acid residues. LAAO imparts yellow color to venom.

애플리케이션

L-Amino Acid Oxidase from Crotalus adamanteus has been used in the quantification of rid protein in imine deaminase activity.

L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.

생화학적/생리학적 작용

L-Amino acid oxidase (LAAO) from Crotalus adamanteus requires Mg²⁺ for its activation. The N-terminal region is essential for FAD binding. The H₂O₂ generated by LAAO based oxidation reactions lead to prevention of platelet aggregation and induces edema and apoptosis. LAAO activity is inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).

제조 메모

Dissolves in water at 1 mg/mL concentration to form a clear solution.

안전성 정보

• 픽토그램: GHS06
• 신호어: Danger
• 유해 및 위험 성명서: H300 + H310 + H330
• 예방조치 성명서: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable