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Merck
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Key Documents

50649

Sigma-Aldrich

Glyoxal solution

for molecular biology, BioReagent, ~40% in H2O (~8.8 M)

동의어(들):

Ethanedial, Oxalaldehyde

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About This Item

Linear Formula:
OHCCHO
CAS Number:
Molecular Weight:
58.04
Beilstein:
1732463
MDL number:
UNSPSC 코드:
12352114
PubChem Substance ID:
NACRES:
NA.52

Grade

for molecular biology

Quality Level

제품 라인

BioReagent

형태

liquid

농도

~40% in H2O (~8.8 M)

불순물

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.409

음이온 미량물

chloride (Cl-): ≤100 mg/kg
sulfate (SO42-): ≤100 mg/kg

양이온 미량물

Ca: ≤5 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤300 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

저장 온도

2-8°C

SMILES string

[H]C(=O)C([H])=O

InChI

1S/C2H2O2/c3-1-2-4/h1-2H

InChI key

LEQAOMBKQFMDFZ-UHFFFAOYSA-N

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일반 설명

Glyoxal is a nucleic acid denaturant. It acts as a specific probe for homopurine strands of DNA.

애플리케이션

Suitable for
  • nucleic acid denaturation
  • binding of DNA/RNA to nitrocellulose membrane

기타 정보

This form of glyoxal is composed of 3 moles of glyoxal and 2 moles of water in a relatively stable configuration.
May precipitate on storage; redissolve at 50-60 °C.

주의사항

May precipitate on storage; redissolve at 50-60 °C.

원리

Glyoxal forms stable complex with guanine residues that results in denaturation of nucleic acids.

관련 제품

제품 번호
설명
가격

픽토그램

Health hazardExclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


시험 성적서(COA)

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문서 라이브러리 방문

이미 열람한 고객

M Vojtisková et al.
Journal of biomolecular structure & dynamics, 5(2), 283-296 (1987-10-01)
Plasmid pEJ4, which is a derivative of pUC19 containing an insert with 60-bp-long homopurine.homopyrimidine tract from sea urchin P. miliaris histone gene spacer, was studied by chemical probes of the DNA structure osmium tetroxide and glyoxal. The former probe reacts
Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
P S Thomas
Methods in enzymology, 100, 255-266 (1983-01-01)
R N Skopp et al.
Analytical biochemistry, 169(1), 132-137 (1988-02-15)
This paper describes agarose gel electrophoresis and silver staining of denatured RNAs. Glyoxal- or formaldehyde-denatured RNAs are electrophoresed in an agarose gel cast on a plastic support using an inert, low conductivity buffer. Following electrophoresis, the gel is stained with
Yu Wang et al.
Chemical Society reviews, 41(11), 4140-4149 (2012-04-18)
Methylglyoxal (MGO) and glyoxal (GO), known as reactive carbonyl species, can be generated endogenously and exogenously (human body and food system). They are attracting increased attention because of their relationship with diabetes and flavour generation. In this review, their characteristics
Fien Van Lancker et al.
Journal of agricultural and food chemistry, 60(18), 4697-4708 (2012-04-03)
Only a minor part of Maillard reaction studies in the literature focused on the reaction between carbohydrates and peptides. Therefore, in continuation of a previous study in which the influence of the peptide C-terminal amino acid was investigated, this study

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