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Merck
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Key Documents

38185

Sigma-Aldrich

Quantification kit for oxidized and reduced glutathione

200 tests

동의어(들):

γ-L-glutamyl-L-cysteinylglycine kit, Quantification kit

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About This Item

UNSPSC 코드:
12352108
NACRES:
NA.32

Quality Level

품질

200 tests

저장 온도

2-8°C

일반 설명

Glutathione Quantification Kit is designed to detect oxidized and reduced Glutathione. Glutathione is usually represented in a reduced form called GSH; however, GSH is converted into its oxidized state (GSSG) upon oxidative stress. The ratio between GSH and GSSG is considered an index for oxidative stress. Glutathione, also known as γ-L-glutamyl-L-cysteinylglycine, is a tripeptide compound that is involved in anti-oxidation and drug metabolism. It acts as a substrate for enzymes like glutathione peroxidase, glutathione S-transferase, and thiol transferase.

애플리케이션

Glutathione Quantification Kit is used to detect the total amount of glutathione as an index for oxidative stress or disease risk.

특징 및 장점

The detection ranges of total glutathione and GSSG using in this kit are from 0.5 μmol/l to 50 μmol/l and from 0.5 μmol/l to 25 μmol/l, respectively.

성분

Glutathione Quantification Kit contains:
  • Enzyme Solution (50µl X 1)
  • Buffer Solution (60ml X 1)
  • Standard GSH (X 1)
  • Masking Reagent (20µl X 1)
  • Co-enzyme (X 2)
  • Substrate (DTNB) (X4)
  • Standard GSSG (X 1)

분석 메모

Glutathione Quantification Kit contains a GSH masking reagent. This enables GSSG detection in the sample by measuring the absorption (λmax = 412 nm) obtained from a colorimetric reaction of DTNB (5,5’-dithiobis (2-nitrobenzoic acid)) coupled with the enzymatic recycling system. The total quantity of GSH can be measured by subtracting the amount of GSSG from the total amount of glutathione.

기타 정보

not available in Korea, Singapore, Taiwan and Japan

픽토그램

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신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

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