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Merck
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Key Documents

I5502

Sigma-Aldrich

Isopropyl β-D-1-thiogalactopyranoside

≥99% (TLC)

동의어(들):

IPTG, Isopropyl β-D-thiogalactoside

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About This Item

실험식(Hill 표기법):
C9H18O5S
CAS Number:
Molecular Weight:
238.30
Beilstein:
4631
EC Number:
MDL number:
UNSPSC 코드:
12352201
PubChem Substance ID:
NACRES:
NA.31

설명

non-ionic

Quality Level

분석

≥99% (TLC)

형태

powder

기술

thin layer chromatography (TLC): suitable

불순물

10.0-22.0% Dioxane

색상

white to off-white

mp

120 -122  °C ((248 - 252 °F ))

solubility

water: 50 mg/mL, clear, colorless

저장 온도

2-8°C

SMILES string

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

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애플리케이션

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

기타 정보

Non-metabolizable galactose analog.
To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

재구성

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

신호어

WarningDanger

유해 및 위험 성명서

Hazard Classifications

Carc. 1B - Eye Irrit. 2

보충제 위험성

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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