62970

Lysozyme from chicken egg white

dialyzed, lyophilized, powder, ~100000 U/mg

• 동의어(들): Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS Number: 12650-88-3
• 효소 위원회 번호: 3.2.1.17 (BRENDA, IUBMB)
• EC Number: 235-747-3
• MDL number: MFCD00131557
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: chicken egg white
• Quality Level: 100
• 형태: powder
• 품질: dialyzed; lyophilized
• 특이 활성도: ~100000 U/mg
• 분자량: single-chain 14.3 kDa; M_r ~14600
• 기술: cell based assay: suitable
• 적합성: suitable for cell lysis
• UniProt 수납 번호: P00698
• 응용 분야: cell analysis
• 저장 온도: 2-8°C
• 유전자 정보: chicken ... LYZ(396218)

설명

애플리케이션

Lysozyme from chicken egg white has been used:

• as a standard in small-angle neutron scattering experiments and infrared absorbance spectroscopy
• as a constituent of cell lysis buffer
• in protein crystallization experiments

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

생화학적/생리학적 작용

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

단위 정의

1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)

기타 정보

Suitable for the hydrolysis of bacterial cell walls, mucopolysaccharides, mucopolypeptides or chitin; degradability of L. lactis subsp. cremoris H2 cell walls; Review

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)